steponeplus realtime pcr system (Thermo Fisher)
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steponeplus realtime pcr system
Steponeplus Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/5%2C6-DIAMINOURACIL+SULFATE/bio_rxiv__2025__10__09__681363-164-18-23
Average 98 stars, based on 1 article reviews
Steponeplus Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steponeplus%E2%84%A2+realtime+pcr+system/5%2C6-DIAMINOURACIL+SULFATE/bio_rxiv__2025__10__09__681363-164-18-23
Average 98 stars, based on 1 article reviews
steponeplus realtime pcr system - by Bioz Stars,
2026-09
98/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Polymerase Chain Reaction:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Software:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the SYBR Green Assay:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Incubation:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Reverse Transcription:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Expressing:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the RNA Extraction:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Reverse Transcription Polymerase Chain Reaction:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Synthesized:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the Derivative Assay:Article Title: Abnormal enterohepatic circulation of bile acids caused by fructooligosaccharide supplementation along with a high-fat diet. Article Snippet: Fructooligosaccharide (FOS) is a widely used prebiotic and health food ingredient, but few reports have focused on its risk to specific populations.. Recently, it has been shown that the intake of inulin, whose main component is FOS, can lead to cholestasis and induce hepatocellular carcinoma in mice fed a highfat diet (HFD); however, the molecular mechanism behind this is not clear.. This study found that FOS supplementation induced abnormal enterohepatic circulation of bile acids in HFD-fed mice, which showed a significant increase in bile acid levels in the blood and liver, especially the secondary bile acids with high cytotoxicity, such as deoxycholic acid. Article Title: Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells. Article Snippet: Briefly, quantitative mRNA expression analysis was performed by quantitative real-time PCR (qRT-PCR) using the Article Title: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination. Article Snippet: The plates were heated in Article Title: Drug Repurposing and Screening for Multiple Sclerosis Targeting Microglia and Macrophages. Article Snippet: Microglia/macrophages (MG/Mφ) play a central role in the pathogenesis of multiple sclerosis (MS).. However, the intricacies of the immunomodulatory microenvironment in MS, particularly the heterogeneity and regulatory mechanisms of MG/Mφ subpopulations, remain elusive.. The commonly used treatment options for MS have several drawbacks, such as significant side effects and uncertain efficacy. Article Title: Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals. Article Snippet: Depression is a multifaceted mental health disorder with complex etiology and significant global burden.. Recent research indicates that the gut microbiota plays a role in the pathophysiology of depression, highlighting the potential role of specific bacterial species in influencing mood and cognitive function.. In this study, we aimed to investigate the presence, copy numbers, and Ct values of selected bacterial species in stool samples from depressed patients (n=50) compared to control subjects (n=50). Article Title: Field assessment of sequential herbicide mixtures on nitrogen cycle-related functions in soybean production Article Snippet: The abundance of AOA and AOB was determined by real-time PCR using a StepOnePlusTM RealTime PCR System with Article Title: Revisiting the follicle-stimulation hormone receptor expression and function in human myometrium and adipose tissue. Article Snippet: One μg of total RNA was incubated for 15 min with Article Title: AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation Article Snippet: RNA extraction and cDNA synthesis were performed as described in Section 2.3, and the cDNA was used as the template for RT-qPCR, which was performed with the |